DIAGNOSTIC METHODS: Permanent Stains (cont.)

6. Why do you have to use the iodine dish and subsequent rinses in your staining set up when staining slides from the proficiency testing agencies (AAB, CAP, various states, etc.)?

7. What role does acetic acid play in the trichrome stain?

8. What causes the xylene (or xylene substitute) dehydration solutions to turn cloudy when a slide from the previous alcohol dish is moved forward into the xylene dish?

9. Why is absolute ethanol (100%) recommended as the best approach?

10. Tips and Pitfalls!