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SPECIMEN
PROCESSING: Ova and Parasite Examination
Why
do you need to perform a permanent stained smear examination
on every stool submitted for an O&P examination?
There
is data to indicate that intestinal protozoa may or may not
always be seen and identified from the concentration examination.
Since the permanent stained smear is designed to facilitate
identification and/or confirmation of the intestinal protozoa,
it is important that this procedure be performed on all stools.
Also, since trophozoite stages will not be visible on the
concentration examination (rare exceptions - trophozoites
can be seen in concentration sediments prepared from SAF-preserved
specimens), it is even more important to examine the permanent
stained smear. Even if organisms (trophozoites and/or cysts)
are seen in the concentration wet mount,
they may
not be identified accurately and may require confirmation
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