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DIAGNOSTIC METHODS: Permanent Stains

What is the purpose of the permanent stained smear?

The purpose of the permanent stained smear is to provide contrasting colors for both the background debris and parasites present; it is designed to allow examination and recognition of detailed organism morphology under oil immersion examination (100X objective for a total magnification of X1000). This examination is primarily designed to allow recovery and identification of the intestinal protozoa.
 
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