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DIAGNOSTIC METHODS: Permanent Stains

Why do you have to use the iodine dish and subsequent rinses in your staining set up when staining slides from the proficiency testing agencies (AAB, CAP, various states, etc.)?

Smears for proficiency testing are prepared from fecal specimens that have been preserved in mercury-based fixatives, so the iodine dish and subsequent rinse steps are required to remove mercury and iodine prior to staining with either trichrome or iron hematoxylin stains.
 
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