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DIAGNOSTIC
METHODS:
Permanent Stains
What
causes the xylene (or xylene substitute) dehydration solutions
to turn cloudy when a slide from the previous alcohol dish
is moved forward into the xylene dish?
If
there is too much water carryover from the last alcohol dish,
the xylene solution may turn cloudy. When this occurs, replace
the 100% alcohol dishes, back up the slide into 70% alcohol
(you can also use a series of steps - 95%, then 70%), allow
it to stand for 15 min and then move the slide forward through
the 100% alcohol steps and xylene steps. |
