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CONCENTRATION:
Procedure Notes (cont.)
- After
the plug of debris is rimmed and excess fluid is decanted,
while the tube is still upside down, the sides of the
tube can be swabbed with a cotton-tipped applicator stick
to remove excess ethyl acetate. This is particularly important
to do if you are working with plastic centrifuge tubes
or the plastic commercial concentrators. If the sediment
is too dry after swabbing the tube, then add several drops
of saline before preparing the wet smear for examination.
- If
there is excess ethyl acetate in the smear of the sediment
prepared for examination, there will be bubbles present
which will obscure the material that you are trying to
see.
4.
If fecal specimens are received in SAF, then begin the procedure
at step 2.
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