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  Macroscopic

CONCENTRATION: Review

Topic Comments
Principle To concentrate the parasites present, either through sedimentation or flotation. The concentration is specifically designed to allow recovery of protozoan cysts, coccidian oocysts, microsporidian spores and helminth eggs and larvae.
Specimen Any stool specimen that is fresh or preserved in formalin, PVA (mercury- or non-mercury-based), SAF, MIF or newer single vial system fixatives.
Reagents 5% or 10% formalin, ethyl acetate, zinc sulfate (flotation procedure only [specific gravity of 1.18 for fresh stool; 1:20 for preserved stool]); 0.85% NaCl; Lugol's or D'Antoni's iodine.
 
  Microscopic
 
Direct Wet Smear
Concentration
Introduction
Formulas
Quality Control
Procedure
Results and Reporting
Procedure Notes
Procedure Limitations
Procedure Review
Tips and Pitfalls
Permanent Stained Smear