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CONCENTRATION:
Review
| Topic |
|
Comments |
| Principle |
|
To
concentrate the parasites present, either through sedimentation
or flotation. The concentration is specifically designed
to allow recovery of protozoan cysts, coccidian oocysts,
microsporidian spores and helminth eggs and larvae.
|
| Specimen |
|
Any
stool specimen that is fresh or preserved in formalin,
PVA (mercury- or non-mercury-based), SAF, MIF or newer
single vial system fixatives. |
| Reagents |
|
5%
or 10% formalin, ethyl acetate, zinc sulfate (flotation
procedure only [specific gravity of 1.18 for fresh stool;
1:20 for preserved stool]); 0.85% NaCl; Lugol's or D'Antoni's
iodine. |
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