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DIRECT
WET SMEAR: Formulas
Saline
(0.85% NaCl)
-
NaCl 0.85 g
- Distilled
water 100.0 ml
1.
Dissolve the sodium chloride in distilled water in a flask
or bottle,
using a magnetic stirrer.
2.
Distribute 10 ml into each of 10 screw?cap tubes.
3.
Label as 0.85% NaCl with an expiration date of 1 year.
4
Sterilize by autoclaving at 121 C for 15 min.
5.
When cool, store at 4 C.
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