MODIFIED IRON HEMATOXYLIN STAIN INCORPORATING CARBOL FUCHSIN STEP: Introduction

The following combination staining method for SAF-preserved fecal specimens was developed to allow the microscopist to screen for acid-fast organisms in addition to other intestinal parasites. For those laboratories using iron hematoxylin stains in combination with SAF-fixed material and modified acid-fast stains for C. parvum, Cyclospora cayetanensis, and I. belli, this modification represents an improved approach to current staining methods. This combination stain provides a saving in both time and personnel use.

Any fecal specimen submitted in SAF fixative can be used. Fresh fecal specimens after fixation in SAF for 30 min can also be used. This combination stain approach is not recommended for specimens preserved in Schaudinn's fixative or PVA.