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  Macroscopic
 
  Microscopic

MODIFIED IRON HEMATOXYLIN STAIN: Procedure

1. Prepare slide.

  1. Place 1 drop of Mayer's albumin on a labeled slide.
  2. Mix the sediment from the SAF concentration well with an applicator stick.
  3. Add approximately 1 drop of the fecal concentrate to the albumin and spread the mixture over the slide.

2. Allow slide to air dry at room temperature (smear will appear opaque when dry).

3. Place slide in 70% alcohol for 5 min.

4. Wash in container (not running water) of tap water for 2 min.

 
 
Direct Wet Smear
Concentration
Permanent Stained Smear
Modified Iron Hematoxylin Stain
  Formulas
Procedure
Notes