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PERMANENT
STAINED SMEAR: Introduction
The detection
and correct identification of many intestinal protozoa frequently
depend on the examination of the permanent stained smear
with the oil immersion lens (100 x objective). These slides
not only provide the microscopist with a permanent record
of protozoan organisms identified but also may be used for
consultations with specialists when unusual morphological
characteristics are found. Considering the morphological
variations that are possible, organisms may be found that
are very difficult to identify and do not fit the pattern
for any one species.
Although an experienced microscopist can occasionally identify
certain organisms on a wet preparation, most identifications
should be considered tentative until confirmed by the permanent
stained slide. The smaller protozoan organisms are frequently
seen on the stained smear when they are easily missed with
only the direct smear and concentration methods. For these
reasons, the permanent stain is |
