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PERMANENT
STAINED SMEAR: Introduction
(cont.)
cysts
fix more easily than mature cysts, and E. coli cysts
require a longer fixation time than do those of other species.
Preparation
of Material for Staining
Fresh
Material
1.
When the specimen arrives, prepare one or two slides with
applicator sticks or brushes and immediately (without drying)
place them in Schaudinn's fixative. Allow the slides to
fix for a minimum of 30 min; overnight fixation is acceptable.
The amount of fecal material smeared on the slide should
be thin enough that newsprint can be read through the smear.
Smears preserved in liquid Schaudinn's fixative should be
placed in 70% alcohol to remove the excess fixative prior
to placement in iodine-alcohol (used for mercury-based
fixatives)
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