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  Macroscopic

PERMANENT STAINED SMEAR: Review (cont.)

Topic Comments
Reagents Trichrome, iron-hematoxylin, modified iron-hematoxylin, polychrome IV, or chlorazol black E stains and their associated solutions; dehydrating solutions (alcohols and xylenes); mounting fluid optional.
Examination Oil immersion examination of at least 300 fields; additional fields may be required if suspect organisms have been seen in the wet preparations from the concentrated specimen.
 
  Microscopic
 
Direct Wet Smear
Concentration
Permanent Stained Smear
Introduction
Trichrome Stain
Iron Hematoxylin Stain
Modified Iron Hematoxylin Stain
Procedure Review