|
TRICHROME
STAIN: Procedure (Mercury-based
Fixatives)
Note:
In all staining procedures for fecal and gastrointestinal
tract specimens, the term xylene will be used in the generic
sense. Xylene substitutes are recommended for the safety
of all personnel performing these procedures.
1.
Prepare slide for staining as described above.
2.
Remove slide from Schaudinn's fixative and place slide in
70% ethanol for 5 min.
3.
Place slide in 70% ethanol plus iodine (strong tea color)
for 1 min for fresh specimens or 5 to 10 min for PVA air-dried
smears.
4.
Place slide in 70% ethanol for 5 min.
|
