TRICHROME STAIN: Procedure (Non-Mercury Based Fixatives)

1. Prepare slide for staining as described above.

2. Place slide in 70% ethanol for 5 min.*

3. Place in trichrome stain for 10 min. Some people prefer to place the dry smear directly into the stain and eliminate step 2 from the protocol.

4. Place in 90% ethanol plus acetic acid for 1 to 3 s. Immediately drain the rack (see Procedure Notes) and proceed to the next step. Do not allow slides to remain in this solution.

5. Dip several times in 100% ethanol. Use this step as a rinse.