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TRICHROME
STAIN: Quality Control
1.
Stool samples used for quality control can be fixed stool
specimens known to contain protozoa or PVA-preserved negative
stools to which buffy coat cells (PMNs or macrophages) have
been added. A quality control smear prepared from a positive
PVA sample or a PVA sample containing buffy coat cells should
be used when new stain is prepared or at least once each
week. Cultured protozoa can also be used. REMEMBER TO
REFER TO CAP CHECKLIST FOR ANY RECOMMENDED CHANGES PER QC
FREQUENCY.
2. A QC slide should be included with each run of stained
slides, particularly if the staining setup is used infrequently.
3. If the xylene becomes cloudy or there is an accumulation
of water in the bottom of the staining dish, discard the old
reagents, clean the dishes, dry thoroughly, and replace with
fresh 100% ethanol and xylene. |
