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  Macroscopic
 
  Microscopic

TRICHROME STAIN: Results and Reporting

Protozoan trophozoites and cysts will be readily seen, although helminth eggs and larvae may not be easily identified because of excess stain retention (wet smears from the concentration procedure[s] are recommended for detection of these organisms). Yeasts (single and budding cells and pseudohyphae) and humans cells (macrophages, PMNs, and RBCs) can be identified. The following quantitation chart can be used for examination of permanent stained smears with the oil immersion lens (100 x objective; total magnification of x 1,000).

Quantitation of parasites, cells, yeasts, and artifacts
   
Quantity No. 10 oil immersion fields ( x 1,000)
Few 2
Moderate 3-9
Many 10
 
 
Direct Wet Smear
Concentration
Permanent Stained Smear
Trichrome Stain
  Formulas
Quality Control
Proc: Mercury Based Fixatives
Proc: Non-Mercury Based Fixatives
Results and Reporting
Notes
Limitations