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TRICHROME
STAIN: Results and Reporting
Protozoan trophozoites and cysts will be readily seen, although
helminth eggs and larvae may not be easily identified because
of excess stain retention (wet smears from the concentration
procedure[s] are recommended for detection of these organisms).
Yeasts (single and budding cells and pseudohyphae) and humans
cells (macrophages, PMNs, and RBCs) can be identified. The
following quantitation chart can be used for examination
of permanent stained smears with the oil immersion lens
(100 x objective; total magnification of x 1,000).
| Quantitation
of parasites, cells, yeasts, and artifacts |
| |
|
| Quantity |
No.
10 oil immersion fields ( x 1,000) |
| Few |
2 |
| Moderate
|
3-9 |
| Many |
10
|
|