PARA-SED™(Illustrated)

- Add 8-10 drops of surfactant to specimen vial, cap securely and shake or vortex.

- Insert Par-Sed™ filtration into specimen vial. Invert complete device and tap on counter top to facilitate filtering.

- Unscrew Para-Sed™ device from 50 ml centrifuge tube.

- Add saline or 10% formalin to red fill line on the 50 ml centrifuge tube, then add ~5 ml of ethyl acetate. Attach screw cap to the tube and vigorously shake.

- Centrifuge for 10 minutes at 500 xg.

- Invert tube to pour off supernatent and debris layer. Ring inside of tube with cotton applicator stick.

- Re-suspend pellet with a few drops of saline or formalin and examine under a microscope.