PARA-SED™ (ILLUSTRATED)
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| 1. Add 8-10 drops of surfactant to specimen vial, cap securely and shake or vortex. | 2. Insert Par-SedÈ filtration into specimen vial. Invert complete device and tap on counter top to facilitate filtering. | 3. Unscrew Para-SedÈ device from 50 ml centrifuge tube. | ||||
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| 4. Add saline or 10% formalin to red fill line on the 50 ml centrifuge tube, then add ~5 ml of ethyl acetate. Attach screw cap to the tube and vigorously shake. | 5. Centrifuge for 10 minutes at 500 xg. | 6. Invert tube to pour off supernatent and debris layer. Ring inside of tube with cotton applicator stick. | ||||
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| 7. Re-suspend pellet with a few drops of saline or formalin and examine under a microscope. | ||||||
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